If you have a CD drive, making a bootable CD from the boot floppy is all you need as long as the boot floppy has enough tools.
I recall I made a bootable MSDOS floppy image with all necessary tools to partition a hard drive, format it, and transfer system files (fdisk, format, sys), and some way to copy rest of the files that go into DOS directory. Depending on what you need, it could be laplink or interlink to transfer files over serial or parallel connection, or terminal software to transfer files with modem protocols like zmodem, or just CD-ROM drivers to unzip from the actual CD-rom volume. Then that floppy image can be used as a bootable floppy or bootable CD-ROM image.
With USB sticks, it's more random if old PCs boot from it, and whether it is seen as removable floppy or fixed disk.