Sadly, vdos32 is a dead project. There seems to be no way to make it work under 64bit versions of Windows, so it is only a few people now who get to use it. When a cpu is running in 32bit mode, there is an on-chip table called the local descriptor table. vdos32 cannot work without access to this and the table doesn't exist when running in 64bit mode.

Sad, and you can't even do exception for only 32 bit? i am WINXP32 bit user, i want it badly to speed up Pyl, of all games that glidos offer i am really interested in Pyl and Blood only. Blood sadly always buggy and it can be wrapped by other apps, or Blood CM project.
I am always looking into Glidos from time to time, in few years. For Pyl i want to buy it, but no advantages since other wrappers do same, only if your win in FPS. Because 5-15 fps is unplayable, and game do not want to run in NTVDM don't know reason =(
Sorry! Your project is amazing, great work!

Maybe this can help you? https://sourceforge.net/p/nxvdm/wiki/Home/