Finally I found a solution using DosBox Daum.
Scenario:
I need to run an application that controls a CNC machine and the application has an issue:
The time that milling motor requires to reach the optimal RPM is timed with a loop instead of timers.
At 486 33MHz works fine but with an Pentium 75MHz appears the issue. The program can be installed in a contemporany computer but the issue is higher.
For that reason I use an old computer without USB support nor network, the only options are CDs, diskettes or null modem.
With DosBox I can control the CPU speed, I'm not familiarized with other VM software.
The solution is to mount the image as another drive on Windows 7 or higher, copy the files and unmount. Then run DosBox.
The next step is to use a shared folder as recomends Jorpho, it's the best option.
Meanwhile I'll use this solution, I wasted too much time to run the original image on a VM. 😠
Thank you for your idea Jorpho 😀