WHY IN THE HELL YOU WANT TO DO THAT? WHY? WHY? WHY? 😖

DOSBOX emulates DOS computer. Including S3 video card. The program you run in DOSBOX has absolutely no idea about your real configuration. It is the point of the emulation - the program sees an old DOS computer (which does not exist physically).
In short - it makes no sense to run there bios updates for your graphic card/motherboard/DVD whatever.
It will not harm anything, but it will not work either. There is not any specific protection as you think. It does not work because it is nonsence. No way around it.

Mirek

Thanks for this topic. It has made my day. 😀

First off, thanks for the quick replies guys! Why do I want to do it? Well.. I wanted to reflash my video card anyways, and there has to be a clever way to do it from windows; thought DOSBox may be a possibility. mirekluza thanks for clarifying exactly what DOSBox does, I appreciate it. As far as there not being any real protection, I'm not sure I agree. The whole point of protected mode is to prevent programs from interfering with others (I know I know the 4GB of addressable memory space too). I knew I could not do it from the command window for this reason; however, I found it interesting that I could at least start Flashrom from DOSBox. I realize that this seems to be an out there and wacky question to begin with, but companies such as MSI have a way to do video bios flashes from Windows (although I haven't actually seen it) and thought DOSBox may also provide a way. At any rate I haven't given up, anybody else with comments and suggestions please feel free to reply, even if to just call me nuts.

To make an analogy of what you are trying to do: Its as if you are trying to replace your real car's engine by replacing a car engine inside of a driving game.