What @DosFreak means is that, if a Glide implementation for DOS doesn't yet exist, and you somehow were able to port the Windows Glide version of the Doom port to run in DOS (depending how "loosely" tied it is to Windows, as @elszgensa mentions), you may or may not have to port any existing FX_ functions used (3Dfx's feature set - bilinear filtering, fog table, z-buffer depth, rasterization ...) to be compatible with the DOS library (glide2x.ovl), assuming there are some differences.
Otherwise, you would be running the Glide implementation, but it would look exactly the same non-Glide, if the features are not implemented for DOS correctly.
The giveaway is the inclusion of an r_glide.dll, which indicates this is specifically for win32 only.
UPDATE: I did some more digging, and found an archive of old Doom Legacy releases, going as far back as version 1.24 (August 1998).
It contains release history on when the 3Dfx Glide support was first introduced in version 1.28 (April 1999):
- 3DFX BETA VERSION
- you can choose between software or 3Dfx accelerated mode
in the Doom Legacy Launcher 'Setup' p […]
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- 3DFX BETA VERSION
- you can choose between software or 3Dfx accelerated mode
in the Doom Legacy Launcher 'Setup' panel, without the
launcher, use "-3dfx" on the command-line
- 3Dfx mode is available through the r_glide.dll, you need
the latest Glide 3 drivers from www.3dfx.com
- uses external r_glide.dll file : this WILL be a feature,
because it allows for future extensions with OpenGL,
Direct3D, or even hi-color software modes
Without looking, based on the text above, the r_glide.dll seems to be their own fork of GLIDE3X.DLL, Glide v3 being win32 only.
DELL Dimension XPS M200s
:Intel P1 MMX 200MHz
:64MB EDO
:DOS 6.22/Win95b
:Matrox Millenium II + m3D (PowerVR PCX2)
Chaintech 7VJL Apogee
:AMD AthlonXP 2700+
:512MB DDR
:Win98SE/2000 SP4
:3dfx Voodoo5 5500 AGP