There is only one PCI slot, hehe.
(The older model, GA-EX58-UD5 did have two, but I no longer own one.)
ETA: I see from my 3DMark records that the Sparkle card did indeed work with the GA-EX58-UD5. The problem is with the newer GA-X58-UD5 rev. 2.0.
ETA2: The card I tested with the EX58 was a SF-PC85GT256U2. I no longer own this card. The two cards I am testing with the X58 are SF-PC85GTT3T256U2. I don't know if this makes a difference.
ETA3: That second number is not a typo. Picture added.
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"This all reminds me when i took the windows vista sticker thingy off my old laptop, and on my washing machine as a joke. A few days later said washing machine stopped working. I still think this cannot be a coincidence."